Journal: The Journal of Veterinary Medical Science

Article Title: Apoptosis in kidney tissue of senior and geriatric cats with chronic kidney disease

doi: 10.1292/jvms.24-0296

Figure Lengend Snippet: Differences in histopathological parameters between the non-azotemic and azotemic chronic kidney disease groups. ( a ) Glomerulosclerosis. ( b ) Interstitial cell infiltration. ( c ) CD34-positive peritubular capillaries. ( d ) Interstitial fibrosis. Mann–Whitney U test. *: P <0.05, **: P <0.01.

Article Snippet: The IHC of 4-HNE and CD34 was performed with following reagents: anti-4HNE rabbit polyclonal antibody (1:50; 4°C overnight; ab46545; Abcam, Cambridge, UK), anti-CD34 goat polyclonal antibody (1:100; 4°C overnight; sc-7045; Santa Cruz Biotechnology, Dallas, TX, USA), biotinylated goat anti-rabbit IgG antibody (1:200; 30 min; Vector Laboratories, Newark, CA, USA), biotinylated rabbit anti-goat IgG antibody (1:200; 30 min; Vector Laboratories), and peroxidase-conjugated streptavidin (ready-to-use; 30 min; Vector Laboratories).

Techniques: MANN-WHITNEY

Journal: The Journal of Veterinary Medical Science

Article Title: Apoptosis in kidney tissue of senior and geriatric cats with chronic kidney disease

doi: 10.1292/jvms.24-0296

Figure Lengend Snippet: Differences in clinicopathological and pathological parameters between the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL)-positive and TUNEL-negative groups in the glomerular cell nuclei. ( a ) Creatinine. ( b ) Glomerulosclerosis. ( c ) Interstitial cell infiltration. ( d ) CD34-positive peritubular capillaries. ( e ) Interstitial fibrosis. ( f ) Cortical 8-hydroxy-2’-deoxyguanosine (OHdG). ( g ) Medullary 8-OHdG. ( h ) Glomerular 8-OHdG. ( i ) Cortical 4-hydroxynonenal (HNE). ( j ) Medullary 4-HNE. No significant differences are detected in any parameters. Mann–Whitney U test.

Article Snippet: The IHC of 4-HNE and CD34 was performed with following reagents: anti-4HNE rabbit polyclonal antibody (1:50; 4°C overnight; ab46545; Abcam, Cambridge, UK), anti-CD34 goat polyclonal antibody (1:100; 4°C overnight; sc-7045; Santa Cruz Biotechnology, Dallas, TX, USA), biotinylated goat anti-rabbit IgG antibody (1:200; 30 min; Vector Laboratories, Newark, CA, USA), biotinylated rabbit anti-goat IgG antibody (1:200; 30 min; Vector Laboratories), and peroxidase-conjugated streptavidin (ready-to-use; 30 min; Vector Laboratories).

Techniques: End Labeling, TUNEL Assay, MANN-WHITNEY

Journal: The Journal of Veterinary Medical Science

Article Title: Apoptosis in kidney tissue of senior and geriatric cats with chronic kidney disease

doi: 10.1292/jvms.24-0296

Figure Lengend Snippet: Correlation between tubular terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling scores and data from clinicopathological and histomorphometrical analyses in the azotemic chronic kidney disease and non-azotemic groups

Article Snippet: The IHC of 4-HNE and CD34 was performed with following reagents: anti-4HNE rabbit polyclonal antibody (1:50; 4°C overnight; ab46545; Abcam, Cambridge, UK), anti-CD34 goat polyclonal antibody (1:100; 4°C overnight; sc-7045; Santa Cruz Biotechnology, Dallas, TX, USA), biotinylated goat anti-rabbit IgG antibody (1:200; 30 min; Vector Laboratories, Newark, CA, USA), biotinylated rabbit anti-goat IgG antibody (1:200; 30 min; Vector Laboratories), and peroxidase-conjugated streptavidin (ready-to-use; 30 min; Vector Laboratories).

Techniques: End Labeling, TUNEL Assay

Journal: Materials Today Bio

Article Title: Neural tissue-engineered prevascularization in vivo enhances peripheral neuroregeneration via rapid vascular inosculation

doi: 10.1016/j.mtbio.2023.100718

Figure Lengend Snippet: Effects of prevascularization on the SCs migration after nerve bridging surgery. The blood vessels by prevascularization in lumens of TENGs promoted the migration of SCs on both sides of nerve trunks. Compared to the control group, as a guiding bridge, the proximal prevascularized neovascularization significantly accelerated the migration of SCs from the proximal stump. (A) A schematic diagram of the prevascularized TENGs for nerve defect bridging. (B) The immunofluorescence images of the longitudinal sections of prevascularized TENGs. The magnified fields at both sides of TENGs were indicated with rectangular frames. The SCs migration and blood vessels were painted. The blood vessels (CD34 positive, green). The SCs (S100 positive, red). The nuclei (Hoechst 33,342, blue). Scale bar, 500 ​μm and 100 ​μm respectively. Histograms of the SCs migration distances at the proximal and distal stumps of TENGs were counted (n ​= ​3). ∗, each group vs. control group. ∗ p ​< ​0.05; ∗∗ p ​< ​0.01; ∗∗∗ p ​< ​0.001; ∗∗∗∗ p ​< ​0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Primary antibodies included goat anti-CD34 antibody (1:50 dilution, R&D), rabbit anti-S100 antibody (1:200 dilution, Abcam), rabbit anti-Ki67 antibody (1:200 dilution, Sigma), mouse anti-NF200 antibody (1:200 dilution, Sigma).

Techniques: Migration, Control, Immunofluorescence

Journal: Materials Today Bio

Article Title: Neural tissue-engineered prevascularization in vivo enhances peripheral neuroregeneration via rapid vascular inosculation

doi: 10.1016/j.mtbio.2023.100718

Figure Lengend Snippet: Effects of prevascularization on the axon extension after nerve bridging. Based on the accelerated migration of SCs, the blood vessels of TENGs by prevascularization significantly enhanced the extension and regeneration of axons. (A) A schematic diagram of the prevascularized TENGs for nerve defect bridging. (B) The immunofluorescence images of the longitudinal sections of prevascularized TENGs at 14 ​d after nerve bridging. The magnified fields were indicated with rectangular frames. The axon extension and blood vessels were illustrated. The blood vessels (CD34 positive, green). The axons (NF200 positive, red). The nuclei (Hoechst 33,342, blue). Scale bar, 500 ​μm and 100 ​μm respectively. Histograms of the axon extension distances at the proximal stumps of TENGs were calculated (n ​= ​3). ∗, each group vs. control group. ∗ p ​< ​0.05; ∗∗ p ​< ​0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Primary antibodies included goat anti-CD34 antibody (1:50 dilution, R&D), rabbit anti-S100 antibody (1:200 dilution, Abcam), rabbit anti-Ki67 antibody (1:200 dilution, Sigma), mouse anti-NF200 antibody (1:200 dilution, Sigma).

Techniques: Migration, Immunofluorescence, Control

Journal: PLoS ONE

Article Title: Characterization of Interstitial Cajal Progenitors Cells and Their Changes in Hirschsprung’s Disease

doi: 10.1371/journal.pone.0086100

Figure Lengend Snippet: ( A–D ) In AP of normal adult colon, the c-Kit positive mature ICCs connected to each other formed extending chords and around AP. ICC progenitors were co-localization of c-Kit + CD34 + Igf1r + found by overlapping fluorescence images. ( E–H ) In the proximal segment of HSCR, c-Kit positive cells and some of c-Kit + /CD34 + /Igf1r + cells were found occasionally. ( I–L ) In the narrow segment of the HSCR colon, it was very difficult to find positive ICC and their network structure was damaged, the c-Kit + /CD34 + /Igf1r + cells could not be located by overlapping fluorescence images. Green, red and pink fluorescence represent c-Kit, CD34 and Igf1r, respectively.

Article Snippet: The primary antibodies were rabbit polyclonal anti-c-Kit (Abcam, 1∶200), goat polyclonal anti-CD34 (Santa Cruz, 1∶200), and mouse monoclonal anti-Igf1r (Abcam, 1∶200).

Techniques: Fluorescence

Journal: PLoS ONE

Article Title: Characterization of Interstitial Cajal Progenitors Cells and Their Changes in Hirschsprung’s Disease

doi: 10.1371/journal.pone.0086100

Figure Lengend Snippet: ( A, B ) The 3D network structures of c-Kit + and c-Kit + /CD34 + /Igf1r + cells were clearly visible in AP of normal adult colon. ( C, D ) The 3D structures in proximal segment of HSCR were not well formed. ( E, F ) The 3D structures in narrow segment of HSCR were completely destroyed.

Article Snippet: The primary antibodies were rabbit polyclonal anti-c-Kit (Abcam, 1∶200), goat polyclonal anti-CD34 (Santa Cruz, 1∶200), and mouse monoclonal anti-Igf1r (Abcam, 1∶200).

Techniques:

Journal: PLoS ONE

Article Title: Characterization of Interstitial Cajal Progenitors Cells and Their Changes in Hirschsprung’s Disease

doi: 10.1371/journal.pone.0086100

Figure Lengend Snippet: Analysis following gating: ( A ) Selection of living mononuclear cells on the histogram with Side Scatter (SSC)/Forward Scatter (FSC). R1 gate was used to select the cells with light scatter properties characteristic of live cells. ( B ) R2 gate was used to select the cells not expressing macrophage markers (F4/80, CD11b) and the general hematopoietic marker CD45 (FITC- cells): gating on histogram SSC/CD45. ( C ) Detection of ICC phenotypes: the c-Kit + cells population was gated in R4 and further analyzed in step E ; the c- Kit low cells population was gated in R3 and further analyzed in next step D . ( D ) The cells in R5 were c- Kit low CD34 + Igf1r + . ( E ) The cells in R6 were c- Kit + CD34 + Igf1r + , and the cells in R7 were c-Kit + CD34 − Igf1r − . ( F ) To confirm the presence of the c-Kit low population (R3) reliably between c-Kit − and c-Kit + populations (R4). Pink discontinuous straight line was used as the dividing line between c-Kit − and c-Kit low populations.

Article Snippet: The primary antibodies were rabbit polyclonal anti-c-Kit (Abcam, 1∶200), goat polyclonal anti-CD34 (Santa Cruz, 1∶200), and mouse monoclonal anti-Igf1r (Abcam, 1∶200).

Techniques: Selection, Expressing, Marker

Journal: PLoS ONE

Article Title: Characterization of Interstitial Cajal Progenitors Cells and Their Changes in Hirschsprung’s Disease

doi: 10.1371/journal.pone.0086100

Figure Lengend Snippet: Upper lane: before sorting, laser confocal microscope displayed phenotypes of ICC progenitor in total cell population from adult normal colon. ICC progenitors showed fluorescence of c-Kit (pink), CD34 (red) and Igf1r (green), merged with blue nuclear stained DAPI. At the same time, other cells only show DAPI staining as negative control, without other fluorescence. Lower lane: after sorting, laser confocal microscopy detected the single intact ICC progenitor with three-color fluorescence. But after immunofluorescence-activated sorting, the fluorescence of ICC progenitors recessed.

Article Snippet: The primary antibodies were rabbit polyclonal anti-c-Kit (Abcam, 1∶200), goat polyclonal anti-CD34 (Santa Cruz, 1∶200), and mouse monoclonal anti-Igf1r (Abcam, 1∶200).

Techniques: Microscopy, Fluorescence, Staining, Negative Control, Confocal Microscopy, Immunofluorescence